Dolichol kinase activity in the developing rat testis
نویسندگان
چکیده
منابع مشابه
Developing and Adult Rat Testis
23 Iron is essential for male fertility and disruptions in iron balance lead to impairment of testicular 24 function. The divalent metal transporter DMT1 is a key modulator of transferrin and non-transferrin 25 bound iron homeostasis. As a first step in determining the role of DMT1 in the testis, we have 26 determined the pattern of DMT1 expression in the developing and adult rat testis. Northe...
متن کاملSaccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity.
The temperature-sensitive Saccharomyces cerevisiae mutant sec59 accumulates inactive and incompletely glycosylated protein precursors in its endoplasmic reticulum at the restrictive temperature. O-mannosylation and glycosyl phosphatidylinositol membrane anchoring of protein are also abolished, consistent with a deficiency in dolichyl phosphate mannose. Membranes prepared from sec59 cells that h...
متن کاملLocalization of dolichol in the lysosomal fraction of rat liver.
The distribution of dolichol and/or dolichol esters in subcellular fractions prepared from a rat liver homogenate has been investigated. After saponification of the various fractions dolichol was isolated and quantitated by high performance liquid chromatography in three systems. The degree of purity of the subcellular preparations was examined by marker enzymes and by electron microscopy. Usin...
متن کاملProtein kinase C modulates ventilatory patterning in the developing rat.
Protein kinase C (PKC) mediates important components of signal transduction pathways underlying neuronal excitability and modulates respiratory timing mechanisms in adult rats. To determine ventilatory effects of systemic PKC inhibition during development, whole-body plethysmographic recordings were conducted in 2-3-d (n = 11), 5-6-d (n = 19), 10-12-d (n = 14), and 20-21-d-old (n = 14) rat pups...
متن کاملPostnatal changes in dolichol-pathway enzyme activities in rat liver.
The activity of hepatic protein N-glycosylation was compared in rats of different ages by incubating UDP-[14C]glucose with liver microsomes. Dolichyl-phosphate [14C]glucose, [14C]glucosyl-oligosaccharide-lipid and [14C]glycoproteins formed were increased after birth to maximal levels at 2 weeks; thereafter dolichylphosphate [14C]glucose remained constant, while [14C]glucosyl-oligosaccharide-lip...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1986
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod34.3.518